Cyclins and CDKs are known to be regulators of cell cycle.
Labelling the
Cyclins and E-cadherin Proteins by Immunohistochemical Analyses
The cell cycle is composed of four phases, including G1, S, G2, and M, and it is regulated by
cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors [15].
Cyclins function as positive regulatory subunits within a class of protein kinases known as cyclin-dependent kinases (CDKs), and have been shown to be important regulators of major cell cycle transitions.
cyclins and cyclin-dependent kinases), cell adhesion molecules, enzymes, and growth factor signaling pathways.
But one of the most important enzymes is a group of two complexes, which act as a cyclic protein-dependent kinase (CDKs) and a group of periodic proteins (
cyclins), which act as a subunit of the subunits and are a kind of very significant silk/threonine protease [6, 7].
The main components of RB pathway involves five family proteins; retinoblastoma tumor suppressor RB- family of pocket proteins (p110, p107, p130), E2F-family of transcription factors (heterodimers of E2F1-8 with DP1-2),
cyclins (D and E-type), cyclin-dependent protein kinases (cdk4, cdk6) and cyclin-dependent kinase inhibitor CDKIs (p16Ink4a, p15Ink4b, p18Ink4c and p19Ink4d).
To investigate whether CA blocked the progression through the cell cycle by inhibiting the levels of the
Cyclins and consequently the activities of CDKs, we first performed immunoblot analysis of CA treated and control PT2 cells with specific antibodies directed against
Cyclin A and B1.
Pestell, "
Cyclins and cell cycle control in cancer and disease," Genes & Cancer, vol.
Cyclins confer substrate specificity and regulation to Cdk/cyclin complexes.