Human dental stem cell derived transgene-free iPSCs generate functional neurons via
embryoid body-mediated and direct induction methods.
The new procedure is an advanced form of the embryonic stem cell test, in which substances are tested in vitro on so-called
embryoid bodies.
3-dimensional cell culture for on-chip differentiation of stem cells in
embryoid body.
Pfx (Invitrogen) was used to amplify gene open reading frames (ORFs) from 10-day
Embryoid Body cDNAs of H1 or cDNAs bought from YouBio (China).
For
embryoid body formation, 1 x [10.sup.6] hADSCs in 15 [micro]l DMEM/F12 supplemented with 2% B27, 20 ng/ml Human Epidermal Growth Factor (H-EGF), 20 ng/ml human basic Fibroblast Growth Factor (hbFGF) suspended on the inner side of tissue culture dishes were cultured for 48 hr under standard conditions.
(2007) found that undifferentiated cell clusters including embryonic stem (ES) cells,
embryoid bodies and mouse 11-day embryos had expression profiles of miRNAs that were simpler than those of mature somatic cells.
Characterization of
embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies.
It has been reported that hESCs first form
embryoid bodies which are basically structured with three germ layers.
Immunofluorescence of hiPSCs,
embryoid bodies (EBs), and hiPSC-derived DA neurons is described in Supplementary Materials and Methods.
Hybrid type N-glycan branches in a transition
embryoid body bear both a2,6- and a2,3- linked Sia in humans (61).