explant

For this reason, the aseptic procedure needs to be able to control contamination without oxidising the explants.

This damage led to ovarian explants that exhibited lower cell number, fewer proliferating cells, increased cell death and the loss of germ cell number, (https://academic.oup.com/humrep/advance-article/doi/10.1093/humrep/dex383/4831088) according to the study .

Therefore, the hypothesis of the present study was that LPS modulates prolactin secretion from the anterior pituitary explants collected from saline- and endotoxin-treated ewes in the follicular phase of the estrous cycle, and this action can be dependent upon the presence of LBP.

Different protocols of surface sterilisation may vary with the type of chosen plant for culture and selected explants to use.

The results showed that various optimal parameters such as 100 mg L-1 of kanamycin concentration for selection of transformants, 2 d of pre-cultivation time, 100 umol L-1 of acetosyringone concentration, 15 min of infection time and 2 d of co-cultivation time were obtained using cotyledonary node explants. The rooting frequency observed on Murashige and Skoog (MS) medium supplemented with 0.2 mg L-1 indole acetic acid and 400 mg L-1 cefotaxime was found to be 100.00%.

A protocol using droplet vitrification technique (Panis, Piette, & Swennen, 2005) was successfully performed using shoot tips as explants (Santos et al., 2015).

Explants were cultured at 37AdegC in a humidified incubator with 5% CO2.

Embryonic callus and bulblet induction medium: Bulb scale explants were transferred to MS (Murashige and Skoog, 1962), Orchimax (Duchefa Biochemie B.V.

The regenerated protocorms from the Phalaenopsis Classic Spotted Pink hybrid were used as explants in this stage; they presented conical shape and green color, and were generated through asymbiotic germination; part of the protocorms was used at the 80th DASI and the other part was used at the 120th DASI.

Regeneration of plants from transformed explants is difficult in genetic transformation protocols, and for recalcitrant species like common bean is one of the unsolved aspects (Liu, Park, Kanno, & Kameya, 2005; Amugune et al., 2011; Collado et al., 2015).

(2005) who tested the 4-minute immersion system every 5 hours and different plant regulators and obtained positive results on the multiplication of the explants, as well as, Debnath (2017), who concluded that the temporary immersion system is efficient for the propagation of this species.