The roots were stained in 0.1% acid
fuchsine solution (acid
fuchsine dissolved in a 1:1:1: mixture of glycerol, lactic acid and distilled water) for 1-3 min prior to counting number of egg masses/root system.
The stigmas of flowers of a plant were excised after 24 h of their pollination and stained with acid
fuchsine for half an hour.
Identification and biotyping of the putative Brucella isolates were done by assessing colony morphology, biochemical reactions, C[O.sub.2] requirement, production of H2S, growth in the presence of the dyes thionine and
fuchsine, reaction with monospecific antisera (A, M, and R), and phage lysis (Wb, Tb, and F25) [8].
Pancreas was removed and fixed in 10 % buffer formalin, and histological slides were prepared and stained with H & E and modified aldehyde
fuchsine method (Noorafshan et al., 2012).
Light microscopy (LM): According to Wodehouse method (1935), pollen samples were taken from plants and slides were prepared by using basic
fuchsine mixture.
FNA material obtained was smeared on slides and directly stained with strong Carbol
fuchsine and steamed for five minutes.
For the histological processing and to avoid any partial spawning, the specimens were anaesthetized during an hour with isotonic magnesium chloride solution (71 g Mg[Cl.sub.2]-6[H.sub.2]O/l) prior to fixation in 10% formaldehyde, embedded in paraffin, sectioned at 10 ([micro]m, and stained with hematoxylin of Carazzi and eosin, and a trichromie staining of hematoxylin of Carazzi: light green, orange G and acid
fuchsine (Gutierrez 1967).
Furthermore,
Fuchsine was used for staining which marked only the nuclei of the cells.
The buccal surfaces of the anterior teeth were examined using a mouthwash of basic
fuchsine as developing agent plaque, and the following numerical scoring system from 0 to 5 was established: 0--there is no plaque; 1 --independent streaks of plaque in the cervical margin of the tooth; 2--a thin continuous band of plaque (up to 1 mm) at the cervical margin; 3--a band greater than one millimeter wide, but covers less than one-third of the crown; 4--the plaque covers a third, but not more than two-thirds of the crown; 5--the plaque covers two-thirds or more of the crown.