The HI has been proposed to distinguish between the two major sources of the WEOM in soils: (i) the SOM, which releases WEOM rather
humified and showing higher HI values and (ii) the fresh material deriving from the microbial cell lysis, characterised by lower HI (Zsolnay et al.
Coarse microaggregates with a diameter of 50-250 [micro]m are unstable under sonification and consist of variably
humified organic residues (L[F.sub.ooc] = L[F.sub.ooc1] + L[F.sub.ooc2]) and organo-clay complexes (Clay).
The humification process is implemented in suspended soils with evident absence of mineral compounds or mineral fine earth, which indicates that humification in conditions of pure organic substrates can result in formation of deep
humified organic matter that is represented by humic acids with an essential aromatic fraction content.
These proteins can be degraded during composting processes and
humified into amino acids and sugars.
Distribution of the
humified fractions and characteristics of the humic acids of an ultisol under cultivation of eucalyptus and sugar cane.
The use of composts as fertilizers has many advantages over the direct application of sewage sludge and livestock effluents to the soil, since composts are stabilized, have
humified organic matter, are free from pathogenic organisms and provide nutrients, including P, to crops (Fuentes et al., 2006; Stutter, 2015).
Pakistan: Khyber Pakhtankhaw, Abbotabad, Ayubia-Khanspur (34deg 4' 0" N, 73deg 24' 0" E), on the ground
humified soil under Abies pindrow-Pinus wallichina thick vegetation, submitted to herbarium, Department of Botany, University of the Punjab, Lahore.
In regard of this, peat deposits consist of two layers: an aerobic upper layer, the acrotelm and the lower, waterlogged, constantly anoxic and usually more
humified layer, the catotelm (Moore 1989).
RPMI-1640 plus GlutaMAX[TM] medium (RPMI) (Gibco/Invitrogen, Auckland, New Zealand) supplemented with 5% human serum and 1% antibiotics (10,000 units/mL penicillin G sodium, 10,000 [micro]g/mL streptomycin sulfate, and 25 [micro]g/mL amphotericin B (Gibco standard medium)) was the culture medium for PBMCs at 37[degrees]C (
humified, 5% C[O.sub.2], in 25 [cm.sup.2] tissue culture flasks (Sarstedt)).
Tissue on the slides was outlined with a DAKO pen (DAKO, Carpinteria, CA, USA) and incubated with primary antibody diluted in blocking solution overnight in a
humified chamber at 4[degrees]C.
This difference is more noted for treatment with sandy soil S2 (Maiz Bachir), this is explained by a low content in
humified organic matter in soils converts into humus thanks to living microorganisms called humificaters and by absence of loamy colloids.
Human cardiomyoblast cells (H9C2) were grown in DMEM medium, supplemented with 10% heat-inactivated bovine serum (Gibco) and lx penicillin-streptomycin (Gibco) at 37[degrees]C in a
humified chamber with 5% C[O.sub.2] supply.