A rapid, non-turbulent flow will decrease the uptake of oxygen and should be the first priority, even if a vacuum is used to evacuate air from the bottles or inert gas is sparged into the bottles.
(3) This stirred, sparged mini bioreactor system is based on the established ambr15 microbioreactor technology and there is published data to show that Chinese Hamster Ovary (CHO) cells cultured in this microbioreactor match those grown in benchtop vessels, making this technology suitable for high-throughput development.
One-liter glass Mason jars each containing a single oyster were filled with artificial Seawater (approximately 20[degrees]C; salinity, 10) and was subsequently sparged with nitrogen gas until the dissolved oxygen level was 0.5 mg/L.
The presence of dissolved oxygen or of sparged air bubbles together with media components, such as transition metal ions including cuprous (Cu+1) or ferrous (Fe+2) ions, can give rise to damaging ROS.
The resulting solution was sparged with bone-dry [C0.sub.2] gas for 45 min, yielding a white precipitate that was isolated by vacuum filtration and washed with hexanes (3 x 5 ml).
The solution is then run off into the copper and the mash tun is "sparged" at a high temperature to extract more sugar and stop the microbiological process.
2005) as follows: approximately 12.5 mg of [C.sub.60] was added to 500 mL of fresh THF in a 1-L amber bottle and sparged with UHP (ultra high purity) nitrogen to remove oxygen.
sparge - scatter with liquid; wet lightly; "Sprinkle the lawn"