Porcine skeletal muscle proteins are hydrolyzed by eight proteases--thermolysin, proteinase K, proteinase E,
ficin, papain, trypsin, a-chymotrypsin, and pepsin--and the ACE inhibitory activities of the hydrolysates have been measured [5].
Briefly, aliquots of 0.5 mL of an overnight culture of the tester strains were diluted in 5 mL of LB medium and then incubated with rigorous agitation in presence of the
ficin substances.
Ficin, an enzyme in figs, is able to metabolize protein into amino acids, which can lead to weight loss.
After harvest, 300 g of fruit for each ripening stage were rinsed in pure water to prevent burning of the fruit by the action of
ficin (enzyme present in the exudate latex after the fruit harvest).
The posthumous publication is sadly inadequate: Jacques Lefevre d'Etaples, Hecatonomiarum libri: Texte latin des Hecatonomies de Lefevre d'Etaples, en parallele avec la traduction latine de Platon par Marsile
Ficin, ed.
Marcile
Ficin, Thelogie platoniciennes de l'Immortalite des Ames, II.
To this mixture was added 1 volume of group O, E antigen-negative, and enzyme (
ficin)-treated reagent RBCs suspended in a 50-g/L NaCl solution.
See also Andre Chastel, Marsile
Ficin et l'Art (Travaux d'humanisme et renaissance, 14), Geneva, 1954.
They are papain from Carica Papaya from Moraceae [20],
Ficin from Ficus carica from Moraceae [8], Calatropin from Calatropis gigantean from Asclepiadaceae [21], Euphorbain from Euphorbia lathyris from Euphorbiaceae [6] and Eravatamin of Ervatomia coronaria [22].