These include the consideration of small sample sizes in various studies, the use of low statistical power, unavailability of accurate measurement assays, and also the biology of exercise itself is affected by a range of factors such as the duration, nature, frequency and the surrounding environmental conditions.15,23 It has also been found that a number of myokines are released in every minute concentrations of
picomole or femtomole in the immediate vicinity of the muscle and therefore they might never reach the circulating body fluids.
O-phthalaldehyde: fluorogenic detection of primary amines in the
picomole range.
PCR reaction was performed in a total of 25 uL reaction mixture with 12.5 was master (Accuprim TM super mix11), 1.5 uL of 10
picomole of each primer, and 1.5 uL extracted DNA as template and 8 uL distilled water.
The 25 uL amplification reaction included 2.5 uL 10x buffer, 2 uL dNTP (2.5 mM each), 10
picomole of each primer, 1.5 U Taq polymerase, 30 ng of genomic DNA and rest milli Q water.
A combination of two types of Thy1 siRNA, 10
picomole of each (cat.
The cDNA was diluted in RNase-free water to working concentration 50 ng for PCR amplification with SCoT primer (20
picomole per reaction).
After the entire procedure a
picomole range of exposure was reached as biomarker for miR.
Results were expressed as reduced NBT
picomole per min per mg of wet tissue.
It was expressed as
picomole of [H.sub.2][O.sub.2] reduced per sec per milligram of protein (pmol of [H.sub.2][O.sub.2]/sec/mg prot).
Detection of
picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay.
The researchers observed a 42% greater risk of periventricular white matter lesion progression for every 50
picomole per liter decrease in vitamin B12.
Schollhorn, "Electrochemically driven release of
picomole amounts of calcium ions with temporal and spatial resolution," Angewandte Chemie, vol.